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Content archived on 2024-04-30

Comparative analysis for the understanding of molecular evolution. Emphasis on gene families, gene duplications and functional restrictions

Objective



The growing number of characterised sequences from the human and other genome projects offer great potentials to understand how gene products are functionally active, constituting the new era of proteome research, including protein evolution with its background in DNA duplications, mutations and rearrangements. All insights can be used in biotechnology to help correct diseases and promote industrial production.
Present project. We apply for an RTD programme, carried out through shared cost actions with a network of four laboratories, with participation, outside the project economy, of a non-EC, fifth laboratory. All laboratories have considerable and joint experience in this type of work and complement each other to represent multi-disciplinary angles. The objectives require two tools: - Sequence data searches with algorithms for alignments, homology assessments, correlations with gene borders, protein structures, and conformational folds. The coordinator and participating departments have demonstrated this capacity in large-scale screenings, establishing protein families, and defining rules for variability in dehydrogenases and other proteins. This involves "in silico" work under area 2.3 in the EC Biotechnology programme.
- Use of the gene families thus obtained, to understand functions of the corresponding products, and to include mutagenesis/expressions and species variants to check conclusions. This involves experimental work under area 2.2 in the EC Biotechnology programme.
Compliance. Since instructions tell that projects in between different areas under the present call will be considered, we hope the touches with both areas 2.2 and 2.3 will be welcomed, especially since analysis of not only entire genomes but also special human genes are mentioned in the instructions for inclusion under area 2.3. The combined use of in silico screenings and experimental verifications gives a unique strength to the coordinated work of all partners.
Short-term goals. Comparisons of known structures will define motifs, which will be used for further screenings, complemented with mutagenesis and molecular modelling to functionally characterise novel forms. The knowledge will give clones, gene products, cell lines, computer programs, databases, and patents, and will allow construction of new products, also for medical applications.
Long-term goals. In collaboration with biotechnological companies we will attempt at achieving large-scale production for disease control and industrial applications.

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Coordinator

KAROLINSKA INSTITUTE
EU contribution
No data
Address
9,Doktorsringen 9 A
171 77 STOCKHOLM
Sweden

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Total cost

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Participants (4)

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