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Map based cloning of agronomically important genes directly from Zea mays.

Objective



Map Maize is designed to develop the procedures required to map base clone agronomically important genes in Zea mays. To demonstrate the feasibility of our approach, we have identified four genes which are of considerable interest to both Europem academic laboratories and plant breeding companies. Although quite different, we intend to identify and characterise each of the four genes using near-identical procedures which will be developed jointly by the eight partners and two sub-contractors in a network of open cooperation. If we are successful, we will have both cloned, via a map based approach, four agronomically important genes and developed, technologies which will find immediate application in the cloning of other genes which are currently the focus of both academic researchers and the major plant breeding companies within the EU.
This ambitious programme will be undertaken by a consortium
consisting of some of the very best European maize scientists from both academia and industry. In addition the consortium will, where necessary, liaise with non-European scientists and non-European initiatives (such as the US National Corn Initiative) to increase the resources at its disposal.
It is important to note that this proposal does not duplicate any work currently being undertaken by US based scientists, on the contrary, because of the currently available resources (for instance the maize YAC library), Map Maize will carry out research which could only be undertaken by a European based consortium.
The four target genes are:
1. Silage Quality Genes
2. The Early Maturity Gene: Mfl
3. The Gametophytic Male Fertility Gene: GaMS-I
4. The Fungal Resistance Gene Complex Rp1
Our strategy does not involve chromosome walking, instead we will use the technique of chromosome landing. In this approach large mapping populations are used in combination with a very large number of molecular markers. Marke.rs which map to the region of interest are then used to screen large insert YAC or BAC libraries. Using this approach, individual clones will hybridize to several markers, with the result that a contiguous fragment of the separate clones can be constructed without the need for chromosome walking. To achieve our goals and to keep the programme highly focused, the work will be organized into 9 separate work packages, with each work package being designed to have its own coordinator, objectives and optimal combination of participants.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

UNIVERSITY OF BRISTOL
Address
Long Ashton Research Station
BS41 9AF Bristol
United Kingdom

Participants (8)

Biocem SA
France
Address
24,Avenue Des Landais
63170 Aubière
CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Spain
Address
18,Jordi Girona 18
08034 Barcelona
LIMAGRAIN Genetics Grandes Cultures S.A.
France
Address

49320 Les Alleuds
Rhône-Poulenc Agrochimie SA
France
Address
14-20,Rue Pierre Baizet
69263 Lyon
SES France S.A.
France
Address
60,Route De Blois
41500 Mulsans
Semences CARGILL
France
Address
Boissay
28390 Toury
THE UNIVERSITY OF MILANO
Italy
Address
Via Celoria 26
20133 Milano
UNIVERSITY OF BOLOGNA
Italy
Address
Via Filippo Re 6-8
40126 Bologna