Objective
Bacillus is the industrial host of choice for the high yield production of bacterial enzymes. A number of problems have hampered the expression of heterologous proteins of industrial interest by Bacillus species. The solution to this problem lies to a large extent in the identification and control of factors affecting (a) genetic stability of cloned genes, (b) the expression of economically relevant genes and (c) the amenability of industrial Bacillus strains to recombinant DNA technology. The main objective is to construct Bacillus strains which are more suitable for industrial use, by exploring the interface between the recent findings in bacterial physiology, functional genomics and the applied biotechnology.
The goal of this project is to optimise Bacillus as a cell factory for the production of proteins of industrial and/or biomedical interest and to characterize bottle-necks detected under fermentation conditions. Our objectives are: i) to minimize the genome size of B. subtilis 168, ii) to analyse the factor(s) that govern the amplification of genetic information and its stability, iii) to characterize the factors that control the metabolic fluxes and iv) to optimize controlled gene expression. We propose to remove dispensable loci of the genome of B. subtilis, to direct the consumption of resources to the production of desired products. We will develop tools to increase controlled gene expression with the aim of optimizing Bacilli as a cell factory. The availability of the entire DNA sequence of the B. subtilis genome will be of great help for these purposes. We will engineer the sugar uptake to minimize the load on the metabolic flux in the manipulated strains. The full exploitation derived from such an analysis will be of direct relevance to the competitiveness of biotechnological companies in the European Union.
The proposed project will have an intensive interaction with two other major EU-sponsored Bacillus projects [Protein secretion (EBSG BIO4 CT96 0097) and Systematic Function Analysis of Bacillus subtilis genes (BSFA, BIO4 CT95 0278). The involvement of some of the participants of these EU-sponsored projects in this proposal guarantees an optimal interaction between the projects and will result in reciprocal benefits.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- medical and health sciences basic medicine physiology
- natural sciences biological sciences genetics genomes
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- engineering and technology industrial biotechnology bioprocessing technologies fermentation
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
28049 MADRID
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.