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Integration at pilot scale of high cell density bioreactor operation with ultra fast on-line downstream processing

Objective



This project aims at validating - at pilot scale - a continuous process whereby heterologous glycoproteins secreted from mammalian cells propagated in continuously stirred tank reactor are extensively purified by an uninterrupted downstream processing.

The original aspects of the Demonstration are:
- integration of high cell density cultivation with ultra fast on-line downstream processing - validation of sonoperfusion at 8L- and 20L culture volume scale - validation of the "high cell concentration per unit culture volume" concept - use of state-of-the art support for Expanded Bed specific Adsorption (EBSA) - use of specific solid phase screening methods for the selection of chromatographic ligands.

Coordinator

Computer Cell Culture Center SA
Address
20,Place Du Parc
7000 Mons
Belgium

Participants (4)

Anton Paar GmbH
Austria
Address
322,Kdrtner Strasse
8054 Graz
Biosepra S.A.
France
Address
Avenue Jean Jaures
92395 Villeneuve La Garenne
Centre National de la Recherche Scientifique
France
Address
Cité Scientifique
59655 Villeneuve-d'ascq
Smithkline Beecham Biologicals S.A
Belgium
Address
Rue De L'institut
1330 Rixensart