Objective
The primary objective is to produce basic knowledge on the mechanisms by which a variety of signals control growth and cell differentiation. The secondary objective will be to develop tools at cellular and molecular levels to render morphogenetic events accessible to scientific analysis.
Several genes encoding ABP-like proteins have been isolated from rice and are under investigation. Tobacco protoplasts from a transgenic cell line have been used to investigate the role of membrane bound factors involved in signal transduction. Antibinding site antibodies blocked neutron activation analysis (NAA) induced gene expression in the protoplasts. Promoter-GUS fusions have been used to investigate the promoters of auxin induced genes and have led to the identification of a region important for auxin induced and root tip specific expression.
The use of 2 phase separation of membranes or additional free flow electrophoresis has demonstrated the association of ABP with plasmamembrane, perhaps via a disulphide link. Iodinated ABP binds to microsomes and plasmamembrane vesicles but not to those from the endomembrane system. Auxin has been shown to modulate the secretion of alpha-amylase by exocytosis.
The FCBP has been isolated and its structural analysis is in progress. There is evidence for the involvement of the phosphoinositide cycle in signal transduction of the fusicoccin effect.
A new electrophoretic method for the separation of hydrophobic proteins has been developed. Using this technique samples of the EBP have been obtained and preliminary amino terminal sequences of subunits obtained. There is no homology with any known protein. Studies on protein kinases indicate that ethylene may modulate the phosphorylation of its own receptor which in turn appears to modulate the affinity of the receptor for ethylene.
The objective of the project is to deepen the understanding of plant development and regeneration by identifying components of transduction chains for plant growth regulators. Four complementary, integrated approaches Will be used as follows:
1. The isolation of genes for the components of perception and transduction chains for auxin, ethylene and fusicoccin, to examine the expression of such genes during development, to manipulate them and to introduce (e.g. antisense) constructs into appropriate host plants (Arabidopsis, tobacco, tomato) in order to probe for function.
2. Using in-vitro reconstitution systems to demonstrate the functionality of identified components of perception and transduction chains and to determine whether interactions occur between components.
3. To use protoplasts to study the functionality of binding proteins for auxin, ethylene and fusicoccin and to determine
Whether interactions are observable, and through the use of impermeant analogues and specific anti-receptor antibodies to determine whether surface or intracellular receptors control similar or separate pathways.
4. To use affinity columns constructed from putative receptors to Separate, purify and identify components of transduction chains.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences chemical sciences electrochemistry electrophoresis
- natural sciences chemical sciences organic chemistry aliphatic compounds
- agricultural sciences agriculture, forestry, and fisheries agriculture grains and oilseeds cereals
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Coordinator
SY23 3DA E Aberystwyth - Dyfed
United Kingdom
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