The primary objective is to produce basic knowledge on the mechanisms by which a variety of signals control growth and cell differentiation. The secondary objective will be to develop tools at cellular and molecular levels to render morphogenetic events accessible to scientific analysis.
Many plant species,including economically important crops,can not be regenerated from single cells in-vitro. We propose to investigate the mechanism controlling the induction of cell proliferation in-vitro. We Will use Brassica napus microspores as a model system since-freshly isolated-microspores can be redirected to an embryo formation pathway at frequencies of up to 70 percent. 32 C heat treatment of an 8 hour duration is sufficient to initiate and sustain the microspore embryogenic process. The B.napus microspore culture system has a number of other characteristics-well suited for such studies. The specific areas to be investigated:genes regulating induction of microspore embryogenesis,regulation of cytoskeletal and other cellular components during the induction phase and inheritance of the induction specific character(s) in a plant population. We will also compare B.napus microspores With Hordeum vulgare(barley) microspores to identify common molecular features-of microspore embryogenesis induction in dicots and monocot. The project proposes to significantly improve our understanding of the inductive phase of microspore embryogenesis in particular and cell proliferation in general. The results Will help us to understand Why microspores of some economically
important plant species can not be induced to undergo embryogenic development in-vitro and open the possibility to manipulate and transfer genes regulating induction of microspore(cell) proliferation to tissue culture non-responsive plant species.
Funding SchemeCSC - Cost-sharing contracts
CB4 4GZ Cambridge
6703 BD Wageningen