Objective
The joint project is to develop a tomato transposon tagging system in order to enable each group to clone different genes. To this end it is aimed to jointly obtain a series of plants each carrying a transposable element insertion (either Ac or a derivative) in different positions that are equally spaced over the twelve chromosomes of tomato and to demonstrate the feasibility of this series for cloning specific genes by targeted transposon tagging.
The project objective is to develop a tomato transposon tagging system in order to clone different genes. It is aimed to obtain a series of plants each carrying a transposable element insertion, either Ac or a derivative, in different positions that are equally spaced over the twelve chromosomes of tomato and demonstrate the feasibility of this series for cloning specific genes by targeted transposon tagging. Initial work was aimed to construct suitable transfer deoxyribonucleic acid (t-DNA) based vectors, obtain transgenic tomato genotypes, and initiate mapping of the t-DNA insertions in a restriction fragment length polymorphism (RFLP) analysis.
Excellent progress has been made towards the objectives. A set of tomato genotypes has been obtained with mapped transposon inserts. Stocks are available with a transposable element for each tomato chromosome. The first transposon tagging experiments in tomato have been initiated, both using linked and unliked transposons. A number of putative mutants have been identified and await further characterization.
The position now exists to tag genes of interest, such as genes involved in disease resistance, hormone biosynthesis and plant cell development.
Transposon insertion has permitted the isolation of genes from bacteria, Drosophila and plants. In tomato, there exists a wealth of genetic variation in genes affecting sexual reproduction, in disease resistance, in the perception or synthesis of plant hormones and genes which are thought to be involved in plant development. In each of the participating laboratories there is interest in one or more of these areas. The project consists of two parts:
developing an efficient transposon tagging procedure in tomato;
tagging desired tomato genes.
The first part is largely additive and is an essential prerequisite to the second part. Each laboratory undertakes to map fifty T-DNA insertions (containing either Ac or Ds ). Furthermore, Ac derivatives will be constructed that provide transposase in trans but can not themselves transpose. This involves fusion of transposase to various constitutive and tissue specific promoters.
During the development of these transposon tagging procedures all four participating groups are sharing the work between them and are exchanging all information and materials. The second part of the project involves tagging of desired genes. These range from disease resistance genes (Cf, Norwich; Asc, Amsterdam) to genes involved in hormone biosynthesis (sitiens, notabilis and flacca, Nottingham) and to genes involved in plant development (Cologne). Clearly, such experiments will be initiated as soon as the stocks are generated in which they can be carried out.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- engineering and technology materials engineering amorphous solids amorphous semiconductors
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences genetics chromosomes
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Coordinator
1081 HV Amsterdam
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.