Objective
The structural and functional characterization of 10 to 15 lipases. The aim of the project is to acquire so much new knowledge about a sufficient number of these enzymes that it will be possible to understand why they are lipases and how they function as such.
Pseudomonas aeruginosa lipase has been purified and enzymatic characterization carried out. A model of the 3-dimensional structure has been prepared using existing lipase structures and amino acid sequence homologies. The amino acid sequence of Bacillus subtilis lipase has been completed and verified. The gene is coding for a precursor lipase molecule consisting of a leader of 32 amino acids and a mature lipase of 184 amino acids. Purification and enzymatic characterization of the lipase has been carried out.
The project aims at elucidating the 3-dimensional structure and the major kinetic properties of lipases from Pseudomonas and Bacillus species. The research involves collaboration between four laboratories in Germany (Professor Winkler/Dr Jager: Ruhr Universitat Bochum), Belgium (Professor Colson: Universite Catholique de Louvain-la-Neuve) and the Netherlands (Dr Misset: Gist-brocades and Dr B. Dijkstra: State University Groningen). From culture filtrates of the mentioned microorganisms (supplied by Misset), the enzymes are purified and (kinetically) characterized (Winkler: Pseudomonas and Colson: Bacillus) and subsequently subjected to crystallization in order to determine the 3-dimensional structure by X-ray crystallography (Dijkstra). Furthermore, samples of the purified lipases will be kinetically studied by the group of Dr Verger (Marseille) using monolayer and oil-droplet techniques.
Site directed mutagenesis will be used in order to identify amino acids which are important for the catalytic function of the lipases.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences earth and related environmental sciences geology mineralogy crystallography
- natural sciences chemical sciences organic chemistry amines
- natural sciences biological sciences microbiology
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Data not available
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
2611 XT Delft
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.