Objective
Many products of major therapeutic value can be obtained only from animal cells grown in culture. The aim of this project is to improve the processes currently employed in this sector of biotechnology with special emphasis on the vectors and promoters used and on the quality of post translational modification.
A large variety of bioactive mammalian proteins is known to have such valuable properties that great scientific effort is invested to find ways for their controlled production in animal cells. Modern cellular and molecular technologies have enabled identification and isolation of the genes that encode such proteins and the corresponding genetic sequences constitute the basic material to genetically engineer animal cells to manipulate production. A number of events are or importance here. For efficient production, proper sorting within a secretory pathway, transport and release into the extracellular environment is required.
In many instances, the primary translation product is a precursor protein that has to be specifically cleaved into a mature bioactive product. Furthermore, post-translational modification (glycosylation, phosphorylation, acetylation, amidation, sulphation, methylation) is often involved in the maturation process of these components. Finally, to ensure high levels of controlled expression in genetically engineered animal cells, appropriate vector systems are to be used. The research programme in this T-project contains the following projects:
Proprotein processing using the novel furin-class of processing enzymes;
Expression of active forms of the furin-like prohormone converting endopeptidases;
Identification of genes involved in sorting, post-translational modification and secretion of peptide hormones;
Development of extrachromosomal vectors for introduction and amplification of foreign genes in animal cells;
Expression of cloned genes into mammalian cells using retroviral- and EBV-derived vectors;
Production of bioactive proteins by animal cells in large scale cell culture;
Processing of precursor proteins for blood clotting factors.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
3000 LOUVAIN / LEUVEN
Belgium
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.