Control of recombinant protein glycosylation under defined cultivation conditions

Project Information

Grant agreement ID: BIOT0304

Project closed

Start date 1 May 1992

End date 30 April 1994

Funded under

• Specific research and technological development programme (EEC) in the field of biotechnology (BRIDGE), 1990-1994

Total cost € 0,00

EU contribution No data

Coordinated by

Gesellschaft für Biotechnologische Forschung mbH
Germany

Objective

Many products of major therapeutic value can be obtained only from animal cells grown in culture. The aim of this project is to improve the processes currently employed in this sector of biotechnology with special emphasis on the vectors and promoters used and on the quality of post translational modification.

The posttranslational modification of biotechnologically produced therapeutic/diagnostic glycoproteins will be studied and compared by applying different fermentation techniques for recombinant mammalian cell lines (BHK-21 cells). Cultivation systems used in animal cell culture technology will be evaluated including: perfused stirred tank and airlift reactor, immurement and entrapment techniques; influence of high density cultures, coated microcarriers; effect of energy sources as well as presence/absence of FCS/protein additives will be controlled.

Secreted products will be purified and the respective carbohydrate structures will be elucidated in detail. At the cellular level, studies will be carried out on the control of cellular nucleotide sugar level, formation of lipid intermediates as well as efficiency of initiation of N-and O-glycosylation. In addition, determination of the activity of cellular glycosyltransferases will allow a detailed description of the effect of different cell culture conditions on the cellular glycosylation machinery which determines the final carbohydrate structure of biotechnologically prepared glycoproteins.

Modelling of culture conditions will be performed leading to controlled posttranslational modification of products in long term culture. A procedure will be established enabling rapid batch analysis of purified glycoproteins with respect to their carbohydrate status ( "oligosaccharide-mapping" ) at the sub-milligram level.

Fields of science

• natural sciences › biological sciences › biochemistry › biomolecules › proteins
• natural sciences › biological sciences › biochemistry › biomolecules › lipids
• natural sciences › biological sciences › biochemistry › biomolecules › carbohydrates
• natural sciences › biological sciences › genetics › nucleotides
• engineering and technology › industrial biotechnology › bioprocessing technologies › fermentation

Programme(s)

• FP2-BRIDGE - Specific research and technological development programme (EEC) in the field of biotechnology (BRIDGE), 1990-1994

Topic(s)

Data not available

Call for proposal

Data not available

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Gesellschaft für Biotechnologische Forschung mbH

• EU contribution: No data
• Address: Mascheroder Weg 1 ; 38124 Braunschweig ; Germany
• Total cost: No data

Participants (1)

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

France

• EU contribution: No data
• Address: Université des Sciences et Technologies de Lille, ; 59655 VILLENEUVE D'ASCQ
• Total cost: No data