Objective
Many products of major therapeutic value can be obtained only from animal cells grown in culture. The aim of this project is to improve the processes currently employed in this sector of biotechnology with special emphasis on the vectors and promoters used and on the quality of post translational modification.
The posttranslational modification of biotechnologically produced therapeutic/diagnostic glycoproteins will be studied and compared by applying different fermentation techniques for recombinant mammalian cell lines (BHK-21 cells). Cultivation systems used in animal cell culture technology will be evaluated including: perfused stirred tank and airlift reactor, immurement and entrapment techniques; influence of high density cultures, coated microcarriers; effect of energy sources as well as presence/absence of FCS/protein additives will be controlled.
Secreted products will be purified and the respective carbohydrate structures will be elucidated in detail. At the cellular level, studies will be carried out on the control of cellular nucleotide sugar level, formation of lipid intermediates as well as efficiency of initiation of N-and O-glycosylation. In addition, determination of the activity of cellular glycosyltransferases will allow a detailed description of the effect of different cell culture conditions on the cellular glycosylation machinery which determines the final carbohydrate structure of biotechnologically prepared glycoproteins.
Modelling of culture conditions will be performed leading to controlled posttranslational modification of products in long term culture. A procedure will be established enabling rapid batch analysis of purified glycoproteins with respect to their carbohydrate status ( "oligosaccharide-mapping" ) at the sub-milligram level.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences biochemistry biomolecules lipids
- natural sciences biological sciences biochemistry biomolecules carbohydrates
- natural sciences biological sciences genetics nucleotides
- engineering and technology industrial biotechnology bioprocessing technologies fermentation
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
38124 Braunschweig
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.