Skip to main content

Targeted inducible amplified homologous expression system for quality products from animal cells in culture (Animal Cell Expression System)

Objective

Many products of major therapeutic value can be obtained only from animal cells grown in culture. The aim of this project is to improve the processes currently employed in this sector of biotechnology with special emphasis on the vectors and promoters used and on the quality of post translational modification.

The task of this project is to:

isolate promoters from CHO which are strong and/or inducible by alterations in the culture conditions;
achieve targeted integration of DNA into a region of the CHO genome which may be amplified;
monitor the glycosylation profile of a protein made in CHO under these different conditions.

At all stages comparisons will be made with the vector host systems currently in use. The project involves both genetic and cell culture methods to achieve the goal of improved productivity from animal cells.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

NATIONAL UNIVERSITY OF IRELAND, GALWAY
Address
University Road
Galway
Ireland

Participants (5)

Dublin City University
Ireland
Address
Glasvenin
9 Dublin
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia
Italy
Address
Via Bianchi 7
25125 Brescia
MRC Collaborative Centre
United Kingdom
Address
1-3 Burtonhole Lane
NW7 1AD London
UNIVERSITA DEGLI STUDI DI PAVIA
Italy
Address
Via Ferrata 1
27100 Pavia
UNIVERSITY OF BIELEFELD
Germany
Address
Universitätsstraße 25
33501 Bielefeld