Many products of major therapeutic value can be obtained only from animal cells grown in culture. The aim of this project is to improve the processes currently employed in this sector of biotechnology with special emphasis on the vectors and promoters used and on the quality of post translational modification.
The task of this project is to:
isolate promoters from CHO which are strong and/or inducible by alterations in the culture conditions;
achieve targeted integration of DNA into a region of the CHO genome which may be amplified;
monitor the glycosylation profile of a protein made in CHO under these different conditions.
At all stages comparisons will be made with the vector host systems currently in use. The project involves both genetic and cell culture methods to achieve the goal of improved productivity from animal cells.
Funding SchemeCSC - Cost-sharing contracts
NW7 1AD London