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Construction of permanently transfected cells expressing steroid hormone receptors

Objective

Many products of major therapeutic value can be obtained only from animal cells grown in culture. The aim of this project is to improve the processes currently employed in this sector of biotechnology with special emphasis on the vectors and promoters used and on the quality of post translational modification.

Steroid hormone receptors are known to be transcriptional activators. They therefore carry the potential to control the expression of target genes. In nature they are expressed at very low levels. In this project a novel approach will be used to increase the level of their expression and indeed the expression of other proteins of interest.
The methods used will involve the transfer to animal cells of the complete transcription mechanism from prokaryotic systems using the lac operon and repressor, the T3 promoter and the T3 RNA polymerase as the expression cassette. In this way the information which is available from prokaryotics on the control of expression and the achievement of high levels of expression will be transferred in one unit into the animal cells. In an extension of this project the cell lines which are developed which over express the steroid hormone receptor will be useful to screen for analogues of steroid hormones which may have therapeutic benefits.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

THE UNIVERSITY OF MILANO
Address
Via Festa Del Perdono 7
20122 Milano
Italy

Participants (2)

NATIONAL RESEARCH COUNCIL OF ITALY
Italy
Address
Via Fratelli Cervi 93
20090 Segrate
RUPRECHT-KARLS-UNIVERSITAET HEIDELBERG
Germany
Address
Seminarstrasse 2
69117 Heidelberg