Objective
A considerable amount of work has been done in recent years on the genetic control of mammalian central nervous system (CNS) development. However, our knowledge of CNS development in general and of rostral CNS specification in particular still remains quite fragmentary.
The proponents set out to adopt a novel methodology based on a recetly devised differential screening technique, to help elucidate some of the molecular mechanisms underlying further subdivision and differentiation of primary brain structures during midgestation brain development.
The aim of the present project is to identify, isolate and analyze novel genes involved at various levels in early brain development, and define their role in normal ontogenesis, and in mutants emerged from the mouse stock or generated by homologous recombination. Our search will not be restricted to genes encoding positional or early patterning information. Instead, the proponents plan to extend our investigation to genes governing various cellular processes. To identify genes displaying restricted, brain-specific or stage-specific expression patterns, the proponents will utilize an updated PCR-based cDNA fingerprinting protocol to analyze tissues obtained from various areas of the brain during early development. The proponents are going to compare patterns obtained from telencephalic vesicles at E10.5 and later stages (temporally restricted expression), as well as patterns from anterior (prosencephalic) and more posterior (mesencephalic) structures at E12.5 (spatially restricted expression). Differentially expressed clones will be sequenced and sequences aligned to online databases through different algorithms (BLAST, FASTA). Six-frame conceptual translations of nucleotide sequences will be analyzed for their coding ability with GRAIL and aligned to protein sequence databases. Multiple alignments and motif searches will be run through the GCG package. Full length clones will be obtained through the screening of custom made mouse embryonic brain libraries; cDNAs will be physically and genetically mapped on the mouse genome; their tissue distribution will be assessed by in situ hybridization of CNS tissue sections. A restricted number of genes of interest will be characterized functionally by a. studies of phenotypic mutants; b. grafting techniques; c. experimental embryology techniques; d. homologous recombination / gene targeting techniques.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences neurobiology
- natural sciences computer and information sciences databases
- natural sciences mathematics pure mathematics mathematical analysis functional analysis
- natural sciences biological sciences genetics nucleotides
- medical and health sciences clinical medicine embryology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
20132 MILANO
Italy
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.