Construction of an integrated transcriptional map of the human X chromosome (3)

Objective

- - To construct a cosmid/PAC/BAC map of the human X chromosome with reference clones accessible to all participants and based on highly annotated YAC maps.
- - To isolate and map the majority of X chromosome transcripts to the cosmide pockets and when established, to the cosmid contigs.
- - To perform large scale sequencing of selected regions of the X chromosome, principally Xq28 and Xp22.

With the availability of near complete coverage of the human X chromosome in YAC contigs, the participants aim at a coordinated effort to construct a map of bacterial vectors, as a substrate to the isolation of most of the transcripts of the chromosome. For both objectives, two different and complementary strategies will be used. First a global and systematic approach will be used by the coordinator. A minimal tiling path of YAC DNA will be isolated by long-range Alu-PCR and screened against a variety of reference cosmid, PAC and BAC libraries, thus constructing a pocket map of the chromosome. A cDNA library enriched for X chromosome transcripts will be constructed by laboratory in Heidelberg and made available to all participants. It will be systematically screened with cosmids from the pocket map to position transcripts. Secondly, a more regional approach will be used by key participants on the X chromosome, who have accumulated large amounts of material and expertise. The same cosmid libraries and selected cDNA library will be made available to all, and this will allow an efficient pooling of results. To this end a core database will be maintained by the coordinator and accessible by all participants through local copies of the same dataset. The complete network will be constructed with the IGD system, based on the ACEDB graphical database program. Large scale sequencing using state of the art technologies and strategies will be performed in areas where sequence ready maps are already available. This will be mainly performed in Xq28 which has been shown to contain an unusually high number of genes, many of which related to genetic disorders.

Programme(s)

• FP4-BIOMED 2 - Specific research, technological development and demonstration programme in the field of biomedicine and health, 1994-1998

Topic(s)

• 5.1 - Gene mapping and analysis

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.

Address

Ihnestrasse 73
14195 Berlin
Germany

Participants (9)

Comitato Promotore Telethon

Italy

Address

Via Olgettina 58
20132 Milano

GERMAN CANCER RESEARCH CENTER

Germany

Address

280,Im Neuenheimer Feld 280
69120 Heidelberg

Institut National de la Santé et de la Recherche Médicale

France

Address

24,Rue Du Faubourg Saint-jacques
75014 Paris

Justus-Liebig-Universität Giessen

Germany

Address

Schlangenzahl 14
35392 Giessen

LUDWIG-MAXIMILIANS UNIVERSITY OF MUNICH

Germany

Address

Goethestrasse 29 Kinderpoliklinik Der Universitat
80336 Munchen

NATIONAL RESEARCH COUNCIL OF ITALY

Italy

Address

Via Guglielmo Marconi 10
80125 Napoli

STICHTING KATHOLIEKE UNIVERSITEIT

Netherlands

Address

10,Geert Grooteplein 10
6500 HB Nijmegen

The Chancellor, Masters and Scholars of the University of Oxford

United Kingdom

Address

South Parks Road
OX1 3QU Oxford

United Medical and Dental Schools of Guy's and St Thomas's Hospitals

United Kingdom

Address

St Thomas's Street London Bridge
SE1 9RT London