Objective
The isolation, purification and evaluation of the first thermal stable alkaline phosphatase to be isolated and purified: its evaluation in DNA based diagnostic tests. DNA based diagnostics are currently limited by the availability of enzyme based signal generation systems. Those available are from mesophillic sources. As a consequence these impose , upon the developed diagnostic tests, and absolute requirement for incorporation of the signal generation component at the end of all the manipulations. This proposal is designed to make available a new generation of signal generation molecules, that will allow incorporation during the procedure thus allowing multiple incorporation of the signal generation molecule. It is proposed that we make available thermostable enzymes, which will enhance both the signal produced as a result of the consequential enhanced kinetics of anu reaction and also allow the incorporation fo the enzymes during the diagnsotic test. DNA based diagnsotic tests reauire that steps are performed at elevated teperatures to allow denaturation and hybridisation to occur. Thermostable enzymes will be resstant ot these steps. In addition to these advantages the currently available enzymes have a limited purity activity and in the case of animal derived alkaline phosphatase, contain DNA and RNA ases which destroy the probe molecules.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences genetics RNA
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
WA16 9PD Knutsford
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.