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Content archived on 2024-04-30

The development of a DNA fungal probe assay for the diagnosis of opportunistic fungal infections in AIDS/Transplant patients

Objective



This proposal aims to develop and test a user friendly kit for the diagnosis and identification of opportunistic fungal pathogens. These are a frequent, and often life-threatening complication in immunocompromised patients e.g. AIDS. Studies show that at least 58-81% of all AIDS patients contract a fungal infection at some stage during their illness, with a mortality rate of 10-20%, and similar incidences and indeed consequences in Transplant patients. Two of the most common opportunistic fungal pathogens prevalent in immunocompromised patients are Aspergillus spp. and Candida spp. Resulting systemic infections incur an extremely high mortality rate and require aggressive therapy. The importance of early diagnosis for effectove therapy, connot be overemphasised. DNA probe technology offers significant advantages over current testing methods in terms of both speed and accuracy. The proposed research will involve the identification and design of specific probes fpr Aspergillus spp. and Candida spp., and the incorporation of the probes into assay formats which will allow the clinician to distinguish between different fungal pathogens. Preparation methods for the extraction of DNA clinical samples will also be examined. After the development of a Kit, it will be necessary to perform extensive clinical trials before its sensitivity and specificity can be guaranteed. Clinical partners will be sought during the exploratory phase to advise on the appropriate formats on the panel of fungal pathogens and to perform phase 1 clinical trials. The partners will include experts in AIDS.HIV and Transplantation fields.

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EAW - Exploratory awards

Coordinator

ARQTECH LABORATORIES LTD.
EU contribution
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Address
Unit 5, Kilkerrin Park 3, Liosbaun Industrial Esta
90 GALWAY
Ireland

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Total cost

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Participants (2)

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