Skip to main content

Transgenic mouse models to study immune tolerance and human disease

Objective

The major scientific goal of this project concerns the induction, maintenance and breakdown of immune tolerance which will be approached using transgenic technology and gene targeting. Understanding the cellular and molecular mechanism which are involved in self tolerance may allow one to influence the process of auto immunity in human disease. Moreover, this knowledge will be invaluable in transplantation rejection, viral diseases and tumor immunology where it is suspected that tumors may induce tolerance in the periphery. All the laboratories involved in this network are already using transgenic technology. A more recently develop technique in this field is the introduction of specific mutations into the mouse germ line by homologous recombination in embryonic stem cells (gene targeting). This approach will rely on the exchange and the interbreeding of the mouse mutants generated by gene targeting and the conventional transgenic technique.
Transgenic and knock out mice for the study of induction, maintenance and breakdown of immune tolerance have been generated. In the first year the various mouse models have been produced and in the second year the mice will be analysed and used as models for human disease. Interdependence between tasks consists in making available to all the network's participants the homologous recombination technology through education and mobility and in sharing transgenic and knock out animals for further development of mouse models for autoimmune diseases.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Università degli Studi di Milano
Address
Via Vanvitelli 32
20129 Milano
Italy

Participants (5)

Institut National de la Santé et de la Recherche Médicale (INSERM)
France
Address
Parc Scientifique De Luminy
13288 Marseille
Institut Pasteur
France
Address
Rue Du Docteur Roux 25
75724 Paris
Roche SpA
Italy
Address
Piazza Durante 11
20131 Milano
Stiftung Deutsches Krebsforschungszentrum
Germany
Address
Im Neuenheimer Feld 280
69120 Heidelberg
UNIVERSITAET KOELN
Germany
Address
Carl-von-linné-weg 101
50829 Köln