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Content archived on 2022-12-23

Herbicide degradation in the subsurface and aquifer environment in Northern and Southern Europe

Objective



Hungary has become the granary of Eastern Europe based upon intensive, specialized large-scale farming. Although the use of fertilizer and pesticide inputs has been reduced during recent years, groundwater. resources remain vulnerable to contamination by nitrate and herbicides. In order to fully evaluate the environmental consequences of these intensive agricultural practices it is necessary to understand the extent to which microbiological processes can contribute to the removal of these potential pollutants in the soil and unsaturated zone.
The University of Reading, Institut de Recherches Chimiques (Belgium) and Institute of Biology and Benaki phytopathological Institute (Greece) are already involved in a project to study herbicide degradation in the unsaturated and saturated zone in Northern and Southern Europe. It is proposed that Debrecen Agricultural University should also become involved in the project in order to extend the studies to Eastern Europe and to consider herbicide degradation and also denitrification
in samples taken from the unsaturated zone.
Two sites will be selected in Hungary at which large pits will be dug in order to gain access to a profile 1.5-2.om deep. Samples of the soil and subsoil material will be taken aseptically from the vertical face of the pits and taken to the laboratory. Subsamples of material will be incubated at 120C with (a) atrazine at relatively high concentrations (to allow detection by HPLC) and (b) nitrate at 50 ppm. Samples will be incubated either aerobically or anaerobically in gas jars and the disappearance of the atrazine and nitrate measured in order to assess the potential for dissipation. Sterile controls will be included to confirm that the processes observed are microbiological. Additional experiments will investigate the effect of addition of acetate on cometabolism of atrazine, and the microbiological characterization of the samples (viable counts and direct microscopy). Fresh samples of material will be transported to the University of Reading for 14C-atrazine degradation studies to measure mineralization, and for 15N-nitrate studies of denitrification.

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Coordinator

UNIVERSITY OF READING
EU contribution
No data
Address
London Road
RG6 6AH READING / SILCHESTER
United Kingdom

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Total cost

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Participants (1)

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