Objective
The additional proposal comes from a laboratory where one of the effector mechanisms of apoptosis, namely induction and activation of the protein cross-linker transglutaminase, has been originally revealed. In the planned studies the Debrecen laboratory intends to clarify: a) which of the presently known signal pathways, involving several oncogene and antioncogene products, of the initiation of apoptosis leads to transglutaminase induction and/or activation, b) whether the induction and activation of the enzyme is directly linked to the action of the protooncogene/antioncogene products, c) which elements (apoptosis response elements?) of the promoter region of the transglutaminase gene participate in the regulation of the apoptosis response and what kind of nuclear proteins/transcription factors (new DNA binding proteins?) are responsible for the apoptotic induction of transglutaminase, d) what are the biochemical consequences of apoptosis, that is what are the downstream events in the biochemical cascade leading to apoptosis with transglutaminase activation.
The Debrecen laboratory is well equipped and prepared for these studies but needs i) the requested funding for carrying out the experiments and ii) an effective collaboration with several of the participating laboratories of the network project "Oncogenes, antisense oligonucleotides and apoptosis" to have the complete set of tools for making a conclusive study. Their joining to the network would benefit both sides providing new reagents, tools, antisense oligonucleotides and methods for the common efforts of the original network project to understand and, hopefully, to influence (for therapeutic purposes) the molecular machinery of apoptosis.
Call for proposal
Data not availableFunding Scheme
CSC - Cost-sharing contractsCoordinator
30 Cork
Ireland