Objective
To evaluate the strengths and weaknesses of two available transgenic rodent mutation assays.
The importance of determining chemically-induced DNA damage and induced mutations in experimental species is well-established. The currently available rodent assays are empirical and do not provide data on the heritability of induced DNA changes. Two commercially available mutation assays utilising the bacterial lac I/Z gene may present a solution to these problems. The two assay systems are the lac Z construct (Mutamouse, Hazelton) and the lac I construct (Big Blue, Stratagene). Both assay systems are currently being refined and the protocols simplified. Close cooperation with Hazelton and Stratagene will ensure that advances in assay protocols will be incorporated into the project as they occur. The main questions to be addressed are:
i) What is the sensitivity and specificity of these assays in respect to carcinogenicity bioassays and established genotoxicity assays, and to what extent is sensitivity chemical and/or tissue specific?
ii) To what extent does the cell type selected from a tissue influence the mutation frequency measured?
iii) Are these assay most usefully employed as acute mutation assays or as models of the process of rodent carcinogenesis at bioassay dose-levels?
iv) What is the relationship between somatic and germ cell mutational effects ?
These studies should enable discernment of the appropriate role in human carcinogenic/mutagenic hazard assessment of the lac I/Z rodent mutation assays. Coordination with studies using the non-transgenic restriction site mutation assays (RSM) will be ensured.
Fields of science (EuroSciVoc)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
SK10 4TJ Macclesfield
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.