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THE PINE WOOD NEMATODE:DEVELOPMENT OF A SAMPLING, EXTRACTION AND IDENTIFICATION METHOD

Objective



Background :

The pine wood nematode, Bursaphelenchus xylophilus, is an important quarantine pest infesting pine trees, thus causing wilt diseases. Serious damage to pine forests has been recorded in Japan and North America. To protect the European forests, directive 77/93/EEC imposes restriction on the importation of soft wood from N-America where this nematode and its vector are widespread.

The objectives are : the development of methods for detection and identification of the pine wood nematode (PWN), Bursaphelenchus xylophilus, in timber and chips.

Short description of the project :

Current methods used in statutory inspection of imported timber need an improvement at three levels : sampling, extraction, identification. The objectives of this project are addressed to perfection of the methodologies practically applyable in the control procedure. Modification of sampling techniques and of methods for extraction/purification of nematode probes from wood is envisaged. For identifications and detectibility of pathogenic nematodes biochemical techniques are to be modified and improved. Finally, Plant Protection Services will be involved in testing the practicability of the new methods.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

MINISTRY OF AGRICULTURE - CENTRUM VOOR LANDBOUWKUNDIG ONDERZOEK-GENT/RIJKSSTATION VOOR NEMATOLOGIE & ENTOMOLOGIE
Address
Burg. Van Gansberghelaan, 96
9820 Merelbeke
Belgium

Participants (2)

CENTRUM VOOR LANDBOUWKUNDIG ONDERZOEK
Belgium
Address
Burg. Van Gansberghelaan, 96
9820 Merelbeke
I.N.R.A.
France
Address
B.p. 2078
06606 Antibes