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NON SPECIFIC SEROLOGICAL REACTIONS IN BOVINE BRUCELLOSIS. DEVELOPMENT OF DIAGNOSTIC TOOLS FOR DIFFERENTIATION BETWEEN BRUCELLOSIS AND INFECTION DUE TO YERSINIA ENTEROCOLITICA O:9

Objective



Background :

Bovine brucellosis (BB) has been eradicated in most parts of Northern EU countries but herds must be monitored continously for the potential presence of the disease. An unacceptable prevalence of the infection persists in certain areas of EU member states. A high level of non-specific reactions to serological tests has recently emerged in several countries. Another bacteria, Yersinia enterocolitica O:9 (YO9), seems to be responsible for these cross-reactions. Unnecessary restrictions, waste of resources, genetic losses, failure to control the disease, as well as trade restrictions are some of the consequences of this situation.

The objective is :

This proposal aims at developing diagnostic tests able to differentiate infection by
Brucella abortus (BA) from infection by YO9. Blood tests based either on cellular or
humoral immunity detecting only the anti-BA response as well as tests to detect
specifically infections due to YO9 will be developed.

Short description of the project :

The project will consist of 7 main tasks:
- Development of a bovine gamma-interferon enzyme immunoassay for Brucella;
- Development of ELISA tests specific to Brucella;
- Development of an indirect ELISA using antigens specific to Yersinia;
- Development of immunoaffinity enrichment to improve direct bacteriological
isolation of Yersinia;
- Development of a polymerase chain reaction-gene probe detection system
specific for pathogenic strains of Yersinia;
- Experimental infection of cattle;
- Field trials.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

CENTRE NATIONAL D'ETUDES VETERINAIRES ET ALIMENTAIRES
Address
Rue Pierre Curie, 22
94703 Maisons-alfort Cedex
France

Participants (7)

ECOLE NATIONAL VETERINAIRE D'ALFORT
France
Address
Av. Du General De Gaulle, 7
94704 Maisons-alfort
FACULTES UNIVERSITAIRES NOTRE-DAME DE LA PAIX
Belgium
Address
Rue De Bruxelles, 61
5000 Namur
I.D. - D.L.O. - INSTITUTE FOR ANIMAL SCIENCE AND HEALTH RESEARCH
Netherlands
Address
Edelhertweg, 15
8219 PH Lelystad
I.N.R.A. - CENTRE DE RECHERCHE DE TOURS
France
Address

37380 Nouzilly
INSTITUT NATIONAL DE RECHERCHES VETERINAIRES
Belgium
Address
Groeselenberg, 99
1180 Bruxelles
MAFF Central Veterinary Laboratory
United Kingdom
Address
Woodham Lane New Haw Addlestone
KT15 3NB Weybridge
RHONE MERIEUX S.A.
France
Address
Rue Marcel Merieux, 254 - B.p. 7009
69342 Lyon