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The evaluation of a simple, cheap rapid method of non-protein nitrogen determination in fish products through the processing/merchandising chain.


(i) validation of P ratio as a reliable and sensitive indicator of fish freshness/spoilage;
(ii) revealing the pattern of evolution of non-protein nitrogenous substances (volatile bases and biogenic amines. in particular) in fish products through typical industrial handling, processing, distribution and storage conditions; and
(iii) developing, methodologies for the determination of volatile bases and biogenic amines which industrial Enterprises and enforcement agencies alike will aspire to use - which, therefore, must be inexpensive and rapid enough to use for on-line quality monitoring as well as being sensitive and reliable.

The project belongs to area 'Quality monitoring and quality assurance for fish products' because it seeks to discover the nature of change with time post-mortem, in parameters for which a long research history (Connell, 1975) has led to their recognition and specification as objective quality indicators in EU legislation.

Task 1

Investigation of the relationship of P ratio (determined by the flow injection/gas diffusion technique and by other accepted standard methods) with sensory index (as determined through organoleptic panel testing) for fish flesh through the post-harvest sequence of handling, processing, storage, distribution and retail display.
Species to be used: cod, sea bream, haddock, Atlantic hake and Mediterranean hake (Partners concentrate on one species each).

Task 2

Adaptation of the FIGD technique developed for free amino acid determination for the determination of biogenic amine concentration in pelagic fish flesh.
Species to be used: sprat, mackerel, herring, sardine and anchovy (Partners concentrate on one species each).

Task 3

Investigation of the post-harvest evolution of biogenic amines in fish flesh subjected to industrially-typical time/temperature/treatment (TTT) histories through handling. Processing, storage, distribution and retail display.
Assessment of effectiveness of FIGD techniques against standard techniques in an industrial situation.

Funding Scheme

CSC - Cost-sharing contracts


Cottingham Road
HU6 7RX Hull
United Kingdom

Participants (4)

Skulagata 4
0101 Reykjavik
Instituto de Investigacao das Pescas e do Mar
Avendida De Brasilia
1449-006 Lisboa
Technological Educational Institution of Athens
Agiou Spiridonos
12210 Egaleo - Athens
Universidad de Barcelona
Av. Joan Xxiii S/n
08028 Barcelona