The objectives of this project are to isolate and characterize cysteine proteinase inhibitors in fish and study the role of these inhibitors in the defence against bacteria and viruses.
Enough primary structure of these inhibitors will be determined either by cloning or direct sequencing for comparison with the mammalian cysteine proteinase inhibitors. The effect and biological mechanism of fish cysteine proteinase inhibitors on apoptosis and other microbiological damage will be investigated. Interesting cysteine proteinase inhibitors will be cloned and expressed as recombinant proteins. The will then be purified and used in biological studies. To investigate the specificity of these inhibitors cysteine proteinases will be isolated. Our hypothesis is that this 'inhibitor system' is a novel non-specific defence system of organisms against infections.
The main goal of our proposal is to elucidate the effects of these proteins and possibly find ways of using the molecules as drugs in aquaculture industries.
Funding SchemeCSC - Cost-sharing contracts