To develop and test microsatellite and mitochondrial DNA markers and to optimise screening conditions to enable high-resolution studies of European lobster genetics.
To determine intra- and inter-population genetic diversity based on microsatellite, mitochondrial DNA and allozyme markers in samples from throughout the range of the European lobster.
To evaluate the use of genetic tagging for determining the contribution of ranched individuals in mixed wild and ranched harvests and to determine the potential genetic impact of stock management and enhancement on natural populations.
To determine the paternity of egg masses from individual females and thus elucidate the breeding structure in various European lobster populations.
To produce a plan for the conservation and management of genetic resources in European lobster fisheries taking into account natural population diversity, extant industry practices, previous and current enhancement activities, and the future potential for farming and or ranching.
METHODOLOGY AND RESEARCH TASKS
The overall methodology relies on the development and optimisation of microsatellite and mitochondrial DNA markers for the European lobster and of the large-scale screening of these markers using manual and automated gel electrophoresis systems. These genetic markers will be used to quantify population genetic variation in samples of lobsters obtained from throughout the geographical range. The genetic markers will also be tested as a means of differentiating between wild and stocked individuals. Microsatellite DNA profiling will be used to determine paternity in egg masses from individual females. Genetic data will be used to produce detailed recommendations for the management of the European lobster resource.
Funding SchemeCSC - Cost-sharing contracts