Objective
The goal of this proposal is to transpose to the fish rhabdovirus the reverse genetic technology which has been used with success for mammalian negative-strand RNA virus like rabies and the vesicular stomatitis virus VSV to generate new live vaccines genetically tagged and cost-effective, deliverable by bath immersion and safe for other fish species
The main objectives for the proposal are:
I Construction of a fully attenuated IHNV strain following targeted mutations:
1. Generation of a full-length cDNA IHNV genome (12 Kbases) as well as individual nucleoprotein, phosphoprotein and polymerase encoding cDNA.
2. Obtaining new infectious IHNV virions following co-transfection and T7-driven vaccinia virus infection in fish cell line.
II Creation of new viral vaccine strain expressing IHN/VHS glycoproteins:
1. Explore the feasibility of the insertion of VHSV glycoprotein encoding gene in the IHN genome using as a potential insertion site a non essential gene (Nv).
2. Evaluation of the efficacy of that new bi-valent live vaccine.
As a first step we have chosen to apply on IHN the reverse genetic technology to produce a safe live vaccine but also to develop a potentially very powerful bivalent live vaccine. If successful, this programme may be the first step of a new generation of fish vaccine of improved efficacy against rhabdovirus disease affecting aquacultured fishes (spring viremia of carp, SVC, perch rhabdovirus disease, PRD ...) but can be used also to fight other viral disease such as IPNV using the rhabdovirus as gene vector.
The improvement in fish protection will play a role in improving health of aquacultured species and consequently on the productivity of fish farms, thus this programme will have a beneficial socio-economic impact, in particular for small-scale fisheries where productivity and profitability are crucial survival conditions.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences health sciences infectious diseases RNA viruses
- agricultural sciences agriculture, forestry, and fisheries fisheries
- natural sciences biological sciences microbiology virology
- medical and health sciences basic medicine pharmacology and pharmacy pharmaceutical drugs vaccines
- natural sciences biological sciences zoology ichthyology
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
78352 JOUY-EN-JOSAS
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.