Factors modifying yield of radiation induced chromosomal aberrations in mammalian cells

Project Information

Grant agreement ID: FI4P960059

Project closed

Start date 1 January 1997

End date 30 June 1999

Funded under

• Specific research and training programme in the field of nuclear safety and safeguards, 1994-1998

Total cost No data

EU contribution No data

Coordinated by

Rijksuniversiteit Leiden
Netherlands

Objective

Ionizing radiation is very efficient in inducing chromosomal aberrations which are associated with human hereditary effects as well as in neoplasia. This proposal is complementary to the already running project (PL950004) and designed to study factors which influence or modify the formation of radiation induced chromosomal aberrations in mammalian cells.
It focuses on intragenomic heterogeneity in radiation induced chromosome aberrations, formation of exchange aberrations and individual susceptibility in radiation response.
To understand the influence of chromatin structure on induction and repair of radiation induced damage, studies will be carried out in Chinese hamster primary cells which have distinct hetero(condensed) and eu-chromatin (open, transcriptionally more active) by using conventional Gbanding and fluorescence in situ hybridization (FISH).
In addition, in human and Chinese hamster cells special emphasis will be given to chromosomes which are extrememly rich in actively transcribed genes and those with limited transcriptional activity by using chromosome specific DNA libraries.
The kinetic of formation of exchange aberrations (unstable, dicentrics; and stable, translocations) in human lymphocytes following treament with ionizing radiation at different stages of cell cycle (Go and G2), different dose levels as well different recovery periods will be investigated using premature chromosome condensation and FISH techniques.
The defects underlying radiosensitivity of cell are variable in many cases and poorly defined.
For chemicals, involvement of several genes (i.e. glutathione transferase) in modifying biological responses have been demonstrated.
Influence of defects in glutathione transferase and N-acetyltransferase genes on sensitivity to ionizing radiation will be studied using different cohorts. The results obtained from this project will give new insights on different aspects of the role of chromatin structure, mechanisms of radiation induced chromosome aberrations and the factors that contribute to the observed yield of aberrations.

Programme(s)

• EAEC-NFS 2 - Specific research and training programme in the field of nuclear safety and safeguards, 1994-1998

Topic(s)

• 040102 - Repair of, and recovery from, DNA damage and radiation sensitivity

Call for proposal

Data not available

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Rijksuniversiteit Leiden

• EU contribution: No data
• Address: 72,Wassenaarseweg ; 2300 RA Leiden ; Netherlands
• Total cost: No data

Participants (1)

Finnish Centre for Radiation and Nuclear Safety

Finland

• EU contribution: No data
• Address: 4,Laippatie ; 00880 Helsinki
• Total cost: No data