Extension of the range of double-stranded dna sequences which can be recognized by oligonucleotides via triple helix formation

Objective

The growing interest raised in olignucleotide-directed triple helix formations ('anti-gene strategy!) is due to their potential biological and therapeutical applications for controling gene expression at both transcriptional and replicational levels. The present project is aimed at developing new approaches to extend the range of double-stranded DNA recognition via oligonucleotide-directed triple helix formation. The ultimate goal is to apply 'antigene strategy' to any DNA target sequence with biological interest without sequence limitation as it is currently restricted to polypurine-polypyrimidine tracts. Syntheses of modified oligonucleotides containing triple helix-specific stabilizers are envisioned. Their abilities to form stable extended triple helices under physiological conditions will be evaluated. Results are expected in inhibition of gene expression in oncogenes and HIV viral genes.

Fields of science

• natural sciences › biological sciences › genetics › DNA
• medical and health sciences › health sciences › infectious diseases › RNA viruses › HIV

Programme(s)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Topic(s)

• 0302 - Post-doctoral research training grants
• TC03 - Biological, Medicinal and Pharmaceutical Chemistry

Call for proposal

Data not available

Funding Scheme

RGI - Research grants (individual fellowships)

Coordinator

Muséum National d'Histoire Naturelle

Address

43,rue Cuvier 43
75231 Paris
France

Participants (1)

Not available

Italy