Objective
In our project we propose to improve the current limitations of quantitative and high sensitive imaging of nucleic acids on solid supports by chemiluminescence. First, using a back illuminated, cooled CCD-camera (charge coupled device), measurements will de- termine the required physico-chemical conditions for quantitative imaging of chemiluminescent probes immobilised on solid supports (membranes) at low light levels. Simultaneously, to reach the ex- treme sensitivity required for certain applications, a second imager, based on an intensified PSD (position sensitive detector), will be developed. In addition, the already patented technology STIC (self triggered intensified CCD), which was developed by the host group, will be implemented in this device. Those modifications are expected to result in the following specifications, to image and quantify, after hybridisation with a non radioactive probe, DNA immobilised on membranes:
? Surface analysed : 20x20 cm2
? Sensitivity : < 3 fg/mm2
? Dynamic range : 5000
? Spatial resolution : < 0.5 mm
Finally, this apparatus will be used in a differential screening experiment on c-jun targets, to test a model of neurobiology. Moreover, molecular genetics in the process of development of Drosophila melanogaster and molecular modifications in the cen- tral nervous system of vertebrates, induced by cocaine and am- phetamines, are planed to be studied.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences neurobiology
- natural sciences biological sciences molecular biology molecular genetics
- natural sciences biological sciences biochemistry biomolecules nucleic acids
- engineering and technology electrical engineering, electronic engineering, information engineering electronic engineering sensors optical sensors
- natural sciences biological sciences genetics DNA
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
91406 ORSAY
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.