The study of recombination between repeated sequences makes possible to understand the processes involved in the generation of deletions and other chromosomal rearrangements.
The cellular functions that stabilize the genome by avoiding recombination between repeated sequences are being identified in Saccharomyces cerevisiae. Hprl, Hrsl and Hrs2 are proteins involved in this stabilization that also play an important role in transcription. They are functionally located at a crossover point between recombination and transcription, likely because they play a role in an element required for both processes. The main objective of this project is to elucidate if this common element is chromatin structure.
We plan to carry out
1. A biochemical characterization of Hprl, Hrsl and Hrs2.
2. An analysis of the influence of hprl, hrsl and hrs2 mutations on chromatin structure.
3. A chromatin-structure analysis of the DNA sequences that are able to initiate homologous recombination in yeast.
4. The initial steps to isolate the elements involved in stabilization of genomic repeated sequences in animals.