Objective
One of the fundamental reactions of carotenoid biosynthesis is cyclization, for example of lycopene to beta-carotene. The accepted mechanism involves initial proton attack on the terminal double bond, ring closure and stabilization by proton loss from C-6, i.e. no change in oxidation level. Recent work has shown that the cyclase has an NADPH- binding site and NADPH is required for the cyclization of lycopene by the isolated enzyme. The mechanism of the reaction will be reexamined and the role of NADPH determined. Samples of NADPH labelled with 3H or 2H will be prepared and used to determine whether NADPH is involve directly in the cyclization and hydrogen incorporated from it into beta-carotene. Structural analogues of NADPH will be examined for inhibitory effects on cyclization, through competitive or non-competitive binding. The effects of modifying or deleting the NADPH-binding site region from the gene for the cyclase will be investigated.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences electrochemistry electrolysis
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
L69 3BX Liverpool
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.