Objective
The primary objectives of the proposed project will be to analyse the role of cytosolic free calcium ([Ca2+]c) in Neurospora crassa for regulating: 1. The initiation of cell polarity (during hyphal branch formation). 2. The maintenance of cell polarity (during hyphal tip growth). 3. The reorientation of cell polarity (during hyphal tip growth in an applied electrical field).
Imaging, quantitation and experimental manipulation of Ca2+ dynamics in living hyphae will be central to the project. The research will involve using:
1. Pressure microinjection of Ca2+-sensitive dyes and caged probes. 2. Confocal imaging of Ca2+-sensitive dyes.
3. Ratio imaging of ([Ca2+]c).
4. Experimental manipulation of Ca2+signalling using photoactiveatable caged probes.
5. Analysis of polarity mutants impaired in chitin synthase and protein kinase activities.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences inorganic chemistry alkaline earth metals
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences cell biology cell polarity
- natural sciences physical sciences optics microscopy confocal microscopy
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
EH9 3Jr EDINBURGH
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.