Objective
The primary objectives of the proposed project will be to analyse the role of cytosolic free calcium ([Ca2+]c) in Neurospora crassa for regulating: 1. The initiation of cell polarity (during hyphal branch formation). 2. The maintenance of cell polarity (during hyphal tip growth). 3. The reorientation of cell polarity (during hyphal tip growth in an applied electrical field).
Imaging, quantitation and experimental manipulation of Ca2+ dynamics in living hyphae will be central to the project. The research will involve using:
1. Pressure microinjection of Ca2+-sensitive dyes and caged probes. 2. Confocal imaging of Ca2+-sensitive dyes.
3. Ratio imaging of ([Ca2+]c).
4. Experimental manipulation of Ca2+signalling using photoactiveatable caged probes.
5. Analysis of polarity mutants impaired in chitin synthase and protein kinase activities.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural scienceschemical sciencesinorganic chemistryalkaline earth metals
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteins
- natural sciencesbiological sciencescell biologycell polarity
- natural sciencesphysical sciencesopticsmicroscopyconfocal microscopy
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Call for proposal
Data not availableFunding Scheme
RGI - Research grants (individual fellowships)Coordinator
EH9 3Jr EDINBURGH
United Kingdom