Objective
SUMMARY
All mammalian mRNAs end with a 200 adenosine residues tail at their 3' ends. Recently several studies have highlighted the role of the poly(A) tail in translation and mRNA stability. A detailed biochemical characterization of the protein components involved in the nuclear polyadenylation have started during last years. Poly(A) polymerase (PAP), necessery for the adenosine addition reaction, has been molecularly cloned from bovine and human. The availability of purified polyadenylation factors and monoclonal antibodies (Mabs) directed against human PAP makes it possible to generate an epitope map of PAP describing the relative location in space of the different epitopes. The epitope map will be related to a linear map which will be obtained by deletion mutagenesis of recombinant PAP followed by Western blot analysis of the various modified PAPs. Comparison of the linear functional maps with the tentative folding map, will allow us to predict a detailed functional/ structural map of PAP. The identification of different epitopes of PAP and their function, will help us to understand the significance of multiple PAPs in mammalian cells.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences organic chemistry organic reactions
- natural sciences biological sciences biochemistry biomolecules proteins
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
751 23 Uppsala
Sweden
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.