Objective
The isolation of the malaria parasite receptor on the mosquito salivary glands, and further assessment of its molecular nature and adhesive characteristics, will give valuable knowledge for the development of strategies to block parasite transmission by the mosquito vector. An An. gambiae adult female salivary gland cDNA expression library will be constructed. The library will then be screened selectively for the plasmodium receptor cDNA by transient expression in COS cells which will be panned (screened) with immobilized hemolymph stage sporozoites. Alternatively, the screening will be done with anti salivary gland monoclonal antibodies, selected for their ability to block parasite invasion of the glands.
The isolated clone will be molecularly characterized and its functional regions mapped, by in vitro and in vivo binding and blocking assays between parasite-receptor and parasite-salivary glands, involving recombinant nested deletions of the receptor and anti receptor antibodies. This project will apart from the knowledge on parasite invasion of mosquito salivary glands also generate a number of tools for the study of vector parasite interactions and vector biology, such as the salivary gland cDNA expression library, a battery of anti salivary gland monoclonal antibodies and standardized binding assays.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences health sciences infectious diseases malaria
- natural sciences biological sciences zoology invertebrate zoology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
69117 Heidelberg
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.