Objective
The transduction of painful stimuli into nerve impulses in pain-sensiti neurones is poorly understood. The proposed project will use cultured neurones from the dorsal root ganglion, which are known to respond to a variety of painful stimuli, and will examine the electrophysiological response of the neurones to bradykinin, capsaicin and noxious thermal stimuli. Techniques to be used will include whole-cell and cell-attached patch clamp, calcium imaging using confocal microscopy, and receptor identification using immunohistochemistry and in situ hybridisation. Stimuli will be applied using a rapid solution change method. The principal projects will include
(i) Biophysical characterisation of ion channels activated by bradykinin, capsaicin and thermal stimuli
(ii) An investigation of signal pathways and internal transmitters underlying activation of ion channels by noxious stimuli
(iii) An investigation of the mechanisms of response adaptation observed on repeated application of noxious stimuli
(iv) An investigation of signal pathways activated by B1 receptors, using neurones from a B2 knockout transgenic mouse.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences inorganic chemistry alkaline earth metals
- natural sciences physical sciences optics microscopy confocal microscopy
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
WC2R 2LS London
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.