Objective
cAMP constitutes a suffficient mitogenic signal for a variety of mammalian cells. The cAMP mitogenic cascade is unique in that it often induces differentiation, in addition to proliferation. However, little attention has been paid to this pathway, as opposed to the better studied tyrosine kinase and protein kinase C cascades.
The present project seeks the cloning and characterization of new protooncogene(s) and/or antioncogene(s) associated to the cAMP mitogenic pathway, using the TSH/thyrocyte model system. The study will be conducted according to the following objectives:
1. Identification of cAMP-regulated genes involved in the TSH mitogenic pathway. This will be performed by differentially screening cDNA libraries from stimulated cells using probes derived from quiescent cells. 2. Sequencing and quinetics of expression of cAMP-controlled cell cycle regulatory genes. Assessment of their role. A regulated, cell-cycle dependent expression will be analized. Sequences of the selected clones will be compared to those of known families.
3. Definition of the role of identified genes in the control of cell proliferation. The protein encoded for by the selected genes wili be expressed in appropiate expression system. Its function will be analyzed in the relevant cell-free systems. Gene expression in normal and malignant tissues will be compared.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
1070 BRUXELLES
Belgium
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.