Objective
Fusion proteins between the hormone binding domain of a mutant mouse oestrogen receptor (ERTM) and the tumour suppressor p53 are strictly tamoxifen-dependent. To study the role of p53 in suppressing carcinogenesis in vivo protein expression of a tamoxifen-dependent domi- nant negative DN p53 ERTM construct will be targeted to specific tissues in transgenic mice. Wild type pS3 will then be functionally inactivated in target tissues by administration of tamoxifen to affected animals and spontaneous carcinogenesis will be awaited or mice will be subjected to various known carcinogenic regimes.
In a second approach, by homologous recombination in ES cells mice will be created in which one copy of wild-type p53 has been replaced with a gene encoding the tamoxifen-de- pendent p53 ERTM protein. The remaining intact p53 allele will be sporadically lost to give rise to tumours which will then be analysed towards their responsiveness to p53 tumour sup- pression by administration of tamoxifen and thereby activating the p53 ERTM protein. These investigations may provide the basis for a successful gene therapy of cancer.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences medical biotechnology genetic engineering gene therapy
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences clinical medicine oncology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
WC2A 3PX London
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.