Objective The ability of Vibrio cholerae to efficiently and selectively secrete cholera toxin represents a remarkable biological process and serves as a paradigm for understanding protein translocation events in bacteria. Cholera toxin is exported to the medium by a two step process which involves crossing the outer membrane as a fully folded protein.The aim of the project is to identify and characterize genes of the toxin secretion apparatus. Some genes involved in the process are homologues of genes in the pul system, found in other Gram-negative bacteria. However, evidence has been obtained that a second translocation machinery is involved. The interactions between the toxin and the proteins of the secretory machinary will be studied, with a focus on the putative outer membrane pore. Comparation between different proteins exported by Vibrio cholerae will be made to determine the similarities in secretion and to identify a secretion signal for translocaiion. Fields of science natural sciencesbiological sciencesmicrobiologybacteriologynatural sciencesbiological sciencesbiochemistrybiomoleculesproteins Programme(s) FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998 Topic(s) 0302 - Post-doctoral research training grants TL02 - Molecular Biology and Biochemistry Call for proposal Data not available Funding Scheme RGI - Research grants (individual fellowships) Coordinator University of Kent at Canterbury Address CT2 2NJ Canterbury United Kingdom See on map EU contribution € 0,00 Participants (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all Not available France EU contribution € 0,00 Address See on map
