Molecular analysis of g-protein coupled receptor kinases following manipulations of serotonergic and noradrenergic transmissions in the rat central nervous system

Project Information

Grant agreement ID: FMBI950509

Project closed

Start date 1 October 1996

End date 30 September 1997

Funded under

• Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Total cost No data

EU contribution No data

Coordinated by

Consorzio Mario Negri Sud - Centro diRicerche Farmacologiche e Biomediche
Italy

Objective

Receptor desensitization is at the origin nf the progressive reduction of efficacy of some drug treatment. Adrenergic (beta2 and alpha2), muscarinic cholinergic, serotonergic (5-HT4) and many other G-protein coupled receptors (GR) are rapidly desensitized when stimulated by an agonist. G-protein coupled receptor kinases (GRK) and beta-arrestins are two families of proteins that are responsible for this process. Despite a better understanding of the molecular mechanisms involving
GRK/beta-arrestins little attention has been paid to the functional roles of these latter proteins.
The aim of our project is to investigates in some rat brain regions, the adaptative changes in GRK/beta-arrestins expression (mRNA and proteins) and activity followlng repeated antidepressant treatment (noradrenergic and serotonergic uptake inhibitor) or specific neural lesion (with 5,7-DHT or DSP-4 neurotoxins). In situ hybridization; Northern blot, rhodopsin phosphorylation assay and quantitative autoradiogaphy shall be used. GRK/,beta-arrestins-induced desensitization could become a new pharmacological targets.

Fields of science

Not validated

• natural sciences › biological sciences › neurobiology
• natural sciences › biological sciences › biochemistry › biomolecules › proteins

Programme(s)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Topic(s)

• 0302 - Post-doctoral research training grants
• TL11 - Neurosciences (inc. Psychology)

Call for proposal

Data not available

Funding Scheme

RGI - Research grants (individual fellowships)

Coordinator

Consorzio Mario Negri Sud - Centro diRicerche Farmacologiche e Biomediche

• EU contribution: No data
• Address: Via Nazionale ; 66030 Chieti - San Maria Imbaro ; Italy
• Total cost: No data

Participants (1)

Not available

France

• EU contribution: No data
• Total cost: No data