Molecular control of human adipose tissue hormone sensitive lipase - hsl - relation with "in vivo" lipolytic activity

Objective

The general aim of the project is to study the cellular and molecular regulation of human adipose tissue HSL in physiology and pathology (obesity, metabolic syndrome), in relationship with the "in vivo" control of lipolysis. This will be achieved using "in vivo" and "in vitro" approaches. In the first part, lipolysis (in the basal state and its response to insulin catecholamines) and therefore HSL activity will be studied by microdialysis of abdominal and gluteal sub-cutaneous adipose tissue. For "in vitro" studies, isolated adipocytes will be obtained from biopsies of sub-cutaneous human adipose tissue from patients and control subjects. HSL response to insulin and A or B adrenergic agents will be measured and the expression of HSL will be determined. Moreover, we shall analyze sequences coding for HSL structure and study the regulation of its expression, looking for point mutations which could explain the effects in HSL function.

Fields of science

• natural sciences › biological sciences › genetics › mutation
• medical and health sciences › basic medicine › pathology
• medical and health sciences › basic medicine › physiology
• medical and health sciences › health sciences › nutrition › obesity

Programme(s)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Topic(s)

• 0302 - Post-doctoral research training grants
• TL02 - Molecular Biology and Biochemistry

Call for proposal

Data not available

Funding Scheme

RGI - Research grants (individual fellowships)

Coordinator

KAROLINSKA INSTITUTE

Address

Karolinska Hospital
17 176 Solna
Sweden

Participants (1)

Not available

France