Objective
One strategy to study folding intermediates is to identify protein fragments or peptide models that fold autonomously. Fragments of proteins having lost some of the tertiary interactions that hold them in contact with other amino acids in the uncleaved protein could mimic the conformation of the early intermediates of folding.
The initial aim of this project is to use a variety of techniques, including hydrogen exchange labelling coupled to NMR, and electrospray mass spectrometry, and combined with stopped-flow methods and NMR experiments, to examine the folding of a variety of protein fragments from two fibrinolytic multidomain proteins: urokinase and streptokinase. Urokinase-type plasminogen activator (u-PA), is a serine protease whose principal function appears to be the activation of plasminogen to plasmin by specific proteolytic cleavage. Streptokinase (SK) is a bactoferial secretory protein and also a potente activator of plasminogen. Both proteins are being widely used as a thrombolytic agents in treatment of acute myocardial infarction. A detailed understanding of domain interactions in these proteins will aid the design of novel therapeutic agents.
Virtually nothing is known about the folding of multidomain proteins. Once the work on fragments of the proteins is complete, studies on the intact proteins can be initiated to explore the mean in which such proteins are assembled.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences chemical sciences organic chemistry amines
- natural sciences chemical sciences analytical chemistry mass spectrometry
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
OX1 3QT OXFORD
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.