Objective 1) Identify the regulatory sequences responsible for the fiber type-specific expression of muscle genes by progressive deletions and site directed mutagenesis. Constructs containing portions of the 5' flanking regions of a gene expressed in slow muscle fibers, (the myosin light chain 2 slow/ventricular gene), and a gene expressed in fast fibers (the MyHC-2X gene), will be linked to reporter genes and used for direct injection of plasmid DNA into fast and slow regenerating skeletal muscles that will be subsequently analyzed. 2)Determine the capacity of skeletal muscle to produce and deliver systemically recombinant serum factors. Appropriate promoter/enhancer combinations are essential for a successful gene therapy. The information obtained from 1) will be used to determine the role of muscle-specific regulatory sequences (alone or with viral promoters/enhancers), in driving the expression of the rat growth hormone gene. This gene will be transfered into muscles of hypophysectomized rats and blood levels of GH and its effects on body growth will be determined. Fields of science medical and health sciencesmedical biotechnologygenetic engineeringgene therapynatural sciencesbiological sciencesgeneticsDNA Programme(s) FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998 Topic(s) 0302 - Post-doctoral research training grants TL02 - Molecular Biology and Biochemistry Call for proposal Data not available Funding Scheme RGI - Research grants (individual fellowships) Coordinator Consiglio Nazionale delle Ricerche (CNR) Address Via loredan 16 35131 Padova Italy See on map EU contribution € 0,00 Participants (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all Not available Spain EU contribution € 0,00 Address See on map
