Objective Determination of the critical protein-protein interactions in transcription systems and in particular between sigma-N and its activator, and the contribution of the sigma-N holoenzyme-activator complex to the DNA strand separation (melting) process are essential for the understanding of the mechanism of transcriptional activation of the RNA polymerase holoenzyme. The sigma-N protein is well suited to analysis as biochemically active domains have been isolated. The overall aims of the proposed work are to: (a) Locate the putative protein-protein interactions between activator and the sigma-N protein, that appear to be critical for activation of RNA polymerase holoenzyme. (b) Identify promoter DNA-contact regions of the sigma-N holoenzyme-activator complex during catalysis of open complex formation. (c) Mutate candidate DNA and activator contact regions of sigma-N identified as described above. Screen mutants defective in either a DNA contact during DNA melting and/or activation interaction. (d) Analyse the DNA-melting process, dependent of the formation of the sigma-N holoenzyme-activator complex. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsnatural sciencesbiological sciencesgeneticsDNAnatural scienceschemical sciencescatalysisnatural sciencesbiological sciencesgeneticsRNA Programme(s) FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998 Topic(s) 0302 - Post-doctoral research training grants TL02 - Molecular Biology and Biochemistry Call for proposal Data not available Funding Scheme RGI - Research grants (individual fellowships) Coordinator IMPERIAL COLLEGE OF SCIENCE, TECHNOLOGY AND MEDICINE Address Campus at silwood park, road a329 (virginia water SW7 2AZ London United Kingdom See on map EU contribution No data Participants (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all Not available Spain EU contribution € 0,00 Address See on map Other funding No data