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Content archived on 2024-05-07

Genetic isolation and molecular analysis of mutations affecting vertebrate eye development

Objective



During the development of the zebrafish eye cells become specified according to their proximo- distal locations. This specification involves hedgehog-related signalling molecules which regulate the allocation of cells to retina or optic stalk. The aim of this project is to genetically isolate mutations that affect this polarity at early stages of eye development.
A F2 screen will be performed and mutants will be pre-selected for necrosis or abnormal eye development. Selected lines will be analysed by in situ hybridisation with apax-6 probe to detect posterior cell fates in the eye at a time prior to the onset of necrosis, enabling us to find mutations which have been neglected in previous screens. Existing as well as new mutants will be analysed at the genetic and molecular level using in situ hybridisation and antibody labelling techniques designed to detect changes spatio-temporal patterning of the developing eye.

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Coordinator

Imperial Cancer Research Fund
EU contribution
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Address
44,Lincoln's Inn Fields
WC2A 3PX London
United Kingdom

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Participants (1)

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