Objective
A study of the organisation of bacterial chromosomes is timely. The complete nucleotide sequences of several bacterial chromosomes have recently been reported, or will become available in the near future. Understanding how these chromosomes are packaged and organised within the cell is a priority for interpreting the new sequence data and their functional implications. It has been suggested that the bacterial chromosome is divided into about 100 independently supercoiled domains although, until now, it has not been possible to study these domains in detail or to assess their functional significance. In collaboration with Professor Higgins' laboratory I have recently developed a highly sensitive and reproducible method for measuring DNA supercoiling in vivo using DNA cross-linking by trimethylpsoralen. Exploitation of this method will allow, for the first time, direct in vivo studies of the supercoiling of individual chromosomal domains, will permit the boundaries of these domains to be mapped, and the effects of transcription ('twin domain' model) and histonelike proteins on DNA supercoiling and domain structure to be assessed. These studies will lead to a much more complete understanding of the organisation and functionality of bacterial chromosomes.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics nucleotides
- natural sciences biological sciences genetics chromosomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
OX3 9DU Oxford
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.