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Content archived on 2024-05-14

Structural and functional analysis of a gene associated with differentiation and transformation in rat brain

Objective



Ethylnitrosourea-induced tumorigenesis of the rat nervous system provides a model for the molecular analysis of cell-type specific carcinogenesis. In the peripheral nervous system, a specific point mutation of the neu (ErbB-2) gene is critically associated with the initiation of malignant transformation in Schwann precursor cells.
In rat brain, the RB13-6 monoclonal antibody recognizes a differentiation antigen transiently expressed by a subset of glial cells highly sensitive to ethylnitrosourea-transformation. The corresponding cell-surface antigen (gpl3RB13-6) is a glycoprotein related to the human and murine plasma cell glycoprotein PC-I. The cDNA and parts of the rat RB13-6 gene have been sequenced. In order to clarify the possible role of gp 130RB13-6 in the tumorigenic process, this project includes: (a) mapping and functional analysis of the 5 regulatory region of the RB13-6 gene; (b) mutation analysis of this gene; and (c) study of the phenotypic changes in cells expressing wild-type or mutant RB13-6 transgenes.

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RGI - Research grants (individual fellowships)

Coordinator

Universität Gesamthochschule Essen
EU contribution
No data
Address
Hufelandstraße 55
45147 Essen
Germany

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Total cost

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Participants (1)

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