Objective
Acute promyelocytic leukaemia (APL), a disease caused by a block in promyelocyte differentiation. is associated with a t(15:17) chromosomal translocation resulting in the production of a hybrid protein, PML-RARa. which consists of a nuclear factor, PML. fused to the retinoic acid receptor a (RARa). The differentiation block is relieved by all-trans retinoic acid (RA), the activating ligand of RARa. Recently it was shown that PML is localized in distinct nuclear bodies and that PML-RARa causes disruption of these bodies together with PML s delocalization into aberrant matrix associated nuclear structures. Treatment of APL cells with RA induces a complete relocalization of PML and restores nuclear bodies. Thus the beneficial role of RA in APL might be related to its ability to restore a normal subnuclear organization. To characterize the normal function of PML-containing nuclear bodies, the major aim of the proposed work is to identify other proteins of these structures. Recombinant PML, ND/SP 100 -another protein component of nuclear bodies- and adenovirus E4ORF 3 products -also interacting with nuclear bodies- will be used as probes for the screening of an expression library to identify proteins interacting with PML. NDtSP 100 or E40RF 3. A yeast interaction trap/two-hybrid system, where PML. ND/SP 100 and E40RF 3 will serve as the "bait" proteins, provides an alternative tool to isolate associated proteins.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences clinical medicine oncology leukemia
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
75724 Paris
France
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