Objective
The RYR1 protein is the main Ca2+ release channel in skeletal muscle SR. Mutations in the gene for this channel are known to cause the inherited syndrome malignant hyperthermia (MH) and make the channel more sensitive to activation by a number of agonists including Ca2+, caffeine, ATP and halothane. In vivo, the RYR1 channel interacts with a number of protein modulators including the irnmunophilin FKBP-12 and calmodulin proteins. In this project I will investigate the effects of RYR1 mutations on interaction of the channel with protein modulators. Specifically, I will use Ca2+ efflux, ligand binding, protein purification, imrnunological detection methods and electrophysiological approaches to perform detailed analysis of calmodulin activation / modulation of, and FKBP 12 interaction with, and modulation of, the normal and mutant RYR1 channels. The results obtained will for the first time provide significant information on the effect of MH mutations on channel modulation by these two associated proteins.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics mutation
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
89081 Ulm
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.