Objective
Site-specific recombination permits highly precise and accurate programmed genetic rearrangements in both prokaryotes and eucaryotes. The Xer site-specific recombination system corrects the potential damage of homologous recombination between circular DNA molecules; homologous recombination can generate dimeric molecules which cannot be segregated efficiently to daughter cells; Xer recombination converts such dimers to monomers. This system uses two related recombinases, XerC and XerD, which catalyse exchanges at a family of sites presents on several plasmids and on the E. coli chromosome. This system appears to be highly conserved amongst bacteria. The aim of this project is to gain further insight into the mechanism of Xer recombination at the chromosomal dif site, and particulary to understand the specific requirements for recombination at this site compared with sites present in naturally occurring plasmids. This study will involve biochemical, genetic and physiological studies. First, the characterization of in vitro recombination reactions using dif substrates and purified recombination proteins will be undertaken. Then site-directed mutagenesis will be used to identify the determinants for dif activity and to study their functional relevance in chromosmal segregation.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics chromosomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
OX1 3QU Oxford
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.