Objective
Transplantation of neural tissues in the central nervous system is widely used to pursue neurobiological issues concerning the normal and pathological brain. Recent developments have opened up the possibilities of grafting primary precursor cells cultured in vitro instead of fetal brain tissues. In that way E10 neural tube of transgenic mice bearing thermolabile TAg of SV40 virus will be dissected in order to isolate new multipotential immortalised neuroepithelial cell lines. These cell lines will be characterised at non permissive temperature using immunocytochemical and nucleic markers for neurons and glia. Selected lines will be transfected with Lac Z reporter gene and microtransplanted into various brain regions. Then the survival, the fate of grafted cells and their capability to restore cognitive function of ischaernied rat will be studied. In addition to provide cell lines of readily expandable cells optimal for recovery of cognitive function, these transplantable cells would be excellent tools to study differentiation, cell plasticity and to introduce in brain exogenous gene.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.
- natural sciencesbiological sciencesneurobiologycognitive neuroscience
- natural sciencesbiological sciencesmicrobiologyvirology
- medical and health sciencesclinical medicinetransplantation
You need to log in or register to use this function
Call for proposal
Data not availableFunding Scheme
Data not availableCoordinator
54006 Thessaloniki
Greece